The “best fit” results from the shape and the amino acid functional group’s attraction to the substrate. Due to this jigsaw puzzle-like match between an enzyme and its substrates (which adapts to find the best fit between the transition state and the active site), enzymes are known for their specificity. This specific environment is suited to bind, albeit briefly, to a specific chemical substrate (or substrates). The unique combination of amino acid residues, their positions, sequences, structures, and properties, creates a very specific chemical environment within the active site. These can be large or small, weakly acidic or basic, hydrophilic or hydrophobic, positively or negatively charged, or neutral. Different properties characterize each residue. Since enzymes are proteins, there is a unique combination of amino acid residues (also side chains, or R groups) within the active site. The location within the enzyme where the substrate binds is the enzyme’s active site. Two reactants might also enter a reaction, both become modified, and leave the reaction as two products. In others, two substrates may come together to create one larger molecule. In some reactions, a single-reactant substrate breaks down into multiple products. There may be one or more substrates, depending on the particular chemical reaction. The chemical reactants to which an enzyme binds are the enzyme’s substrates. They only reduce the activation energy required to reach the transition state ( (Figure)).Įnzyme Active Site and Substrate Specificity
This is because they do not change the reactants’ or products’ free energy. In other words, they do not change whether a reaction is exergonic (spontaneous) or endergonic. It is important to remember that enzymes do not change the reaction’s ∆G. Enzymes do this by binding to the reactant molecules, and holding them in such a way as to make the chemical bond-breaking and bond-forming processes take place more readily. Almost all enzymes are proteins, comprised of amino acid chains, and they perform the critical task of lowering the activation energies of chemical reactions inside the cell. Discuss enzyme regulation by various factorsĪ substance that helps a chemical reaction to occur is a catalyst, and the special molecules that catalyze biochemical reactions are enzymes.Explain how enzymes function as molecular catalysts.Describe the role of enzymes in metabolic pathways.By the end of this section, you will be able to do the following: